Abstract:The chromosomes from root tips of Lilium leucanthum(Baker) Baker were analyzed by Giemsa C-banding. The result showed that, the karyotype is 2n=2x=24, the banding number of one genome was 21.The C-banding karyotype would be: 2n=24=6C+2CI+2I+2CI++2CI++4I++2I++2T++2I+S. Significantly different C-banding points were shown on each chromosome. Most of the strong bands of L. leucanthum were distributed on the centromeric regions of the chromosomes. Each chromosome of L. leucanthum(Baker) Baker can be distinguished by Giemsa C-banding.