Abstract:Meiosis of megaspore and karyottype were studied in Longiflorum-Asiatic Lily “Fangio”. the results showed that Meiosis of megaspore of lily “Fagio” is abnormal, the chromorome number of lilium“Fagio” is 2n=3x=36,the chromorsome composition is R(2n) =3x=R(2n) =3x=12m(SAT) +3sm+3sm(SAT) +12st+3st(SAT) +3t.There are subsidiacy bodies on the second,fourth,eighth,ninth,tenth,twelfth belongs pair of chromorome. Its karyoty is 3B type in Taxonomy. Scales and plantlet in vitro of Lily “Fangio” was treated with colchicine at different concentrations and time. The highest induction frequency (20%) some of them are sextuploid.