Abstract:The megasporogensis and female gametophyte formation in Berberis pruinosa were first studied through paraffin sectioning. The main results can be concluded as follows: solitary pistil is monocarpellary with 2 ovules, anatropous，marginal placenta, crassinucellate. The zig-zag micropyle is formed by both the inner and the outer integuments. Single archesporial cell is under nucellar epidermis. The tetrad of megaspores is linear shaped, with the first cell from the chalazal end being functional. The development of embryo sac belongs to the Polygonum type. In mature embryo sac, two polar nucleus fuse into the secondary nucleus before fertilization, three antipodal cells are ephemeral. Inside the triangular egg apparatus, two synergids with fifiform apparatus are developed.