Abstract:In order to improve biological control efficacy of Bacillus subtilis to apple anthracnose, by means of bioassay method, the mechanisms of action and efficacy of Bacillus subtilis strain BS80-6, screened by ion implantation, against apple anthracnose caused by Colletotrichum gloeosporioides were studied in vitro and on apples, in different conditions. The results showed that BS80-6 was more effective against apple anthracnose. An application of a cell suspension (108 cells per mL) of the antagonist in artificial wounds of apples reduced growth of C.gloeosporioides after storage at different temperatures. The inhibitory actions of cell cultures to mycelium growth and to spores germination were 80.14% and 98.65%, respectively, and the control efficacy with cell cultures to the disease was 60.34% at room temperature. The cell culture of BS80-6 provided better control of apple anthracnose than culture filtrates and autoclaved cells. The store temperatures could affect the control efficacy, disease lesion expansion and formation of acrvulus, significantly. There was better control efficacy in fruits inoculated with spores after BS80-6 than before BS80-6.