Abstract:In the course of screening for novel naturally occurring fungicides from mushrooms in Yunnan Province, China, the ethanol extract of the fruiting bodies of Daldinia concentria was found to show antifungal activity against plant pathogenic fungi. The active compounds were isolated from the fruiting bodies of D.concentria by bioassay-guided of the fractionation of the extract and identified as L-696, 474 and cytochalasin D by IR, 1H and 13C NMR and mass spectral analysis. Their antifungal activities were evaluated in vitro against 10 plant pathogenic fungi and in vivo against the plant disease of Erysiphe graminis. In vitro, R.solani and Sclerotinina sclerotiorum were the most sensitive to L-696, 474,and their mycelial growth inhibitions were 83.5% and 82.9% at 10μg/mL, respectively. Rhizoctonia solani, Gaeumannomyces graminis, S.sclerotiorum, Fusicladium pyrnum, Gloeosporium fructigenum were the most sensitive to cytochalasin D, and their mycelial growth inhibitions were 83.6%, 82.4%, 81.9%, 80.5% and 79.7% at 10μg/mL, respectively. Spore germination of G.fructigenum was almost completely inhibited by 200μg/mL for L-696, 474 and cytochalasin D. In vivo, the curative effects of L-696, 474 and cytochalasin D on E.graminis were 74.3% and 85.7% at 500μg/mL, respectively, after 10 days. The preventive effects of L-696, 474 and cytochalasin D on E.graminis were 67.1% and 79.5% at 500μg/mL, respectively, after 10 days.