Abstract:In order to find out whether the microsatellite loci screened from European Cydia pomonella populations are available for microsatellite markers in Chinese C.pomonella populations, the amplifying stability and genetic diversity of 12 microsatellite loci were investigated based on eight geographic populations of codling moth including 120 individuals collected from mainly distributed regions of China. It was observed that eight of the 12 selected loci could be successfully amplified through polymerase chain reaction (PCR). Most populations revealed departure from Hardy-Weinberg equilibrium and the mean number of alleles ranged from 3.750 to 12.500 at each loci. The mean value of observed heterozygosity (Ho) was between 0.025 and 0.783, and the excepted heterozygosity (He) was between 0.284 and 0.892. The lowest value of observed heterozygosity and highest value of inbreeding coefficient (Fis) were observed at locus Cp4.56 (Ho: 0.025; Fis: 0.954), followed by Cp2.P (Ho: 0.109; Fis: 0.806), sug- gesting significant heterozygote deficiencies at these two loci. The other six microsatellite loci showed re- latively higher heterozygosity and larger amount of alleles and thus could be considered usable in research of microsatellite markers for Chinese C.pomonella populations.