Abstract:The high quality flyblow chitin and flyblow chitosan were extracted from flyblow using optimizing processes of calcium removal of EDTA and reaction system of sodium hydroxide-pentanol with two times microwave heating respectively, was used to induce Trichoderma atroviride T2 produced more chitinase. The activity of chitinase by induced T.atroviride T2 were also determined and the antagonistic effects of induced T.atroviride T2 on four pathogenic fungi were detected. The results showed that the output ratio of flyblow chitin and the ash content extracted by using this optimized technology reached 75.0% and 17.3%. The high quality chitosan which contained 95.5% deacetylating degree of chitosan and 8.36% water content were obtained. Sodium hydroxide dosage was used less and reaction time shortened from 4h to 6min. The chitinase activity of T.atroviride T2 cultured in flyblow chitin medium was obviously higher than others cultured in PDB, SCMC and chitosan mediums, which were 0.9721U/mL, 0.4565U/mL, 0.7665U/mL and 0.7315U/mL, respectively. In different mediums, the optimum time for chitinase production in T.atroviride T2 was 120h and the highest activity of chitinase was 1.8340U/mL. The inhibition effects of T.atroviride T2 cultured in flyblow chitin medium antagonized on four pathogenic fungi, were higher than control.