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期 刊 ：植物保护学报 2008年

关键词：毛竹枯梢病；竹喙球菌；拮抗细菌；巨大芽孢杆菌；芽孢；

Keywords:top blight of bamboo, Ceratosphaeria phyllostachydis, antagonistic bacterium, Bacillus megaterium, spore,

摘 要 ：为了提高芽孢的形成率,采用单因素和正交试验设计,通过摇瓶培养对毛竹枯梢病拮抗细菌巨大芽孢杆菌6-59菌株芽孢形成的发酵培养基和培养条件进行优化。结果表明:优化后的培养基配方为:玉米粉1.0%、大豆蛋白胨1.0%、CaCl₂ · 2H₂O 0.1%、MnSO₄ · H₂O 0.05%;适宜培养条件是:种龄20h、接种量8%、培养基初始pH为6.0、装液量30mL/250mL、培养温度30℃、摇床转速200r/min。在此优化条件下,6-59菌株发酵液中的芽孢数量为2×10⁹个/mL,芽孢形成率达96.4%。

Abstract:In order to improve the formation rate and the quantity of the spore, the medial components and cultural conditions of antagonistic strain 6-59 of Bacillus megaterium against Ceratosphaeria phyllostachydis were optimized by single factor and orthogonal tests in shake-flask fermentation method. The results showed that the optimal medial components were composed of 1.0% corn meal, 1.0% soybean tryptone, 0.1% CaCl₂ · 2H₂O, 0.05% MnSO₄ · H₂O. The optimal culture conditions were at temperature 30℃ with initial pH 6.0 and incubation time 20 h, inoculum volume 80 mL/L, medium volume 30mL in 250mL flask and rotation speed 200r/min. Under this optimum conditions, the spore biomass achieves 2×10⁹cfu/mL, formation rate achieves 96.4%.

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