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期 刊 ：植物保护学报 2011年

关键词：苘麻；

Keywords:Alternaria augustiovoide, Echinochloa crus-galli, tenuazonic acid, lipoxygenases, lipid peroxidation,

摘 要 ：苘麻Abutilon theophrastii Medic.为锦葵科苘麻属草本植物,其全草含芸香甙,根含粘液质,苘麻子含15％~17％脂肪油和球蛋白,可治赤白痢疾、痈肿、关节酸痛等症,有消炎、利尿、下乳等作用。烟草花叶病毒Tobacco mosaic virus寄主范围广、侵染性强,可侵染茄科、十字花科、葫芦科及豆科等植物。作者等2008年进行药用植物病害调查时,发现山西晋中地区许多苘麻叶片表现出褪绿、坏死斑等症状,对苘麻药用成分的含量、制药的质量和疗效存在潜在影响。为此,本研究通过血清学、双链RNA(double-stranded RNA,dsRNA)分离和RT-PCR等检测技术,对侵染苘麻的病原进行了鉴定。

Abstract:In order to know the action of tenuazonic acid (TeA) produced by Alternaria augustiovoide strain AAEC05-3 on Echinochloa crus-galli (L.), the effect of TeA on lipid peroxidation of E.crus-galli leaf was studied by leaf segment method in vitro. The result showed that low concentration TeA did not affect the membrane permeability and lipid peroxidation of E.crus-galli leaf, but the membrane permeability and lipid peroxidation increased significantly in 24 h after treatment with high concentration of TeA. When E.crus-galli leaves were treated with 100μmol/L TeA, the relative conductivity and malondialdehyde (MDA) content increased 28.21% and 63.58%, respectively. After the treatment of 100μmol/L TeA, lipoxygenases (Lox) activity of leaf increased 61.59%, meanwhile, the content of unsaturated fatty acids decreased dramatically. The content of linoleic acid and linolenic acid decrease 19.56% and 17.20% in 100μmol/L TeA treatment, respectively. After the treatment of 100μmol/L TeA, the fatty acid hydroperoxides (FAH) was largely accumulated in E.crus-galli leaf. The results indicated that TeA could induce the lipid peroxidation of E.crus-galli leave. It is deduced that the lipid peroxidation induced by TeA could be Lox-mediated lipid peroxidation.

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