Abstract:Strawberries production was damaged severely by Strawberry mottle virus (SMoV). In order to improve the stability and sensitivity of detection of SMoV, we attempted to detect SMoV by transcriptional enhancement techniques. The primers were designed in the conservative non-coding region of the 3‘ end of SMoV genome. The primers with T7 promoter sequence were applied in reverse transcription-polymerase chain reaction (RT-PCR). The transcription in vitro for PCR products was brought into effect by T7 RNA polymerase, and then the products were separated by electrophoresis in agarose gels. The results showed that SMoV could be detected steadily by transcriptional enhancement techniques. It was more sensitive than standard RT-PCR. Both were good when the T7 promoter sequence was added sense primer or antisense primer.