Abstract:Temperature and host growth are considerable factors to affect the overwinter process of the wheat stripe rust pathogen, Puccinia striiformis. The multi-year (1960-2001) and multi-location weather data were used to determine the probability of average minimal temperature in December or January greater than -7℃(P) for each location. The regional classification for the pathogen overwinter in China was conducted with wheat-growth information and GIS technology. The study demonstrated that P values as 70%-85% can be used to determine the borderline of pathogen overwinter to classify the regions. This borderline primarily consistent to that of previous studies can be described as Daxing County (Beijing) -Xushui(Hebei Province) -Yangquan (Shanxi Province) -Jiexiu (Shanxi Province) -Yanchang (Shaanxi Province) -Qingyang-Pingliang-Lixian (Gansu Province)-Songpan (Sichuan Province)-Maerkang (Sichuan Province). The regions in south and east of the borderline are suitable to pathogen overwinter. The results has some comparability and the differentia is that north overwinter line is not Dezhou, Shandong Province, but Beijing.