Abstract:Xiaoyan 6 is an elite wheat cultivar with high-temperature resistance to stripe rust, developed by using exogenous genes from Elytrigia elongata at the end of 70’ s last century. Inheritance of stripe rust resistance gene in Xiaoyan 6 is important for revealing mechanism of resistance to stripe rust and developing durable cultivar. In controlling temperature (20-22 ℃), seedlings of Xiaoyan 6, Mingxian 169 and their F2 progeny were tested with Chinese races (CY29, CY30, CY31, CY32 and Su-4) of Puccinia striiformis f.sp. tritici. The results indicated that the resistance of Xiaoyan 6 to CY31, CY29 and Su-4 is controlled by one dominant gene and one recessive gene, and two complementary dominant genes to CY32 and CY30 in Xiaoyan 6. Xiaoyan 6 as resistant resource and its genetic information should be useful in breeding durable resistant cultivar to stripe rust.