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作 者 ：徐晶;朱茂祥;陈婉华;杨陟华;谢应先;龚诒芬

期 刊 ：植物营养与肥料学报 1997年 3卷 2期 页码：169-175

关键词：亚硒酸钠；螺旋藻；同化作用；75Se示踪；

Keywords:Sodiumselenite, Spirulina, Assimilation, 75Setracer,

摘 要 ：以亚硒酸钠做为螺旋藻富硒的来源，研究了亚硒酸钠梯度浓度对钝顶螺旋藻（Spirulinaplatensis）生长及其富硒量的影响，并采用⁷⁵Se标记的Na₂SeO₃示踪方法，对富硒螺旋藻含硒成分进行了研究。结果表明，当硒浓度超过360μg／mL时，螺旋藻的生长即受抑制，硒浓度为46μg／mL时，表现出促进螺旋藻生长的趋势，而硒浓度为184μg／mL时，螺旋藻可达到最高富硒量，平均为659.9ng／mg。富硒螺旋藻中结合在大分子上的有机硒达到70％以上，其中主要是含硒蛋白质，含量可达50％以上，主要以硒代蛋氨酸和硒代半胱氨酸形式存在，分别占含硒蛋白的40％和20％。

Abstract:Sodiumm selenite, as a nutrient source of selenium was used to culture spirulina and the influence of gradient concentration of sodium selenite on the growth of Spirulina platensis and selenium enrichment content had been researched. Moreover,the selenized component of selenium-enriched spirulina was studied with the tracer technique of ⁷⁵Se labeling Na₂SeO₃. The results showed that the growth of spirulina was inhibited as the selenium concentration beyond 360μ g/mL. Otherwise, trend of low selenium concentration (46μ g/mL) promoting the growth of spirulina was also showed.The highest selenium content of spirulina reached 659. 9ng/mg on an average. while the selenium concentration in medium was 184μ g/mL. In the selsnium-enriched spirulina,the content of organic selenium which were connected on the macromolecules amounted over 70%. the main selenized protein reached over 50%, seleno-methionine and selenocysteine accounted for 40% and 20% of selenized protein respectively.

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