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作 者 ：李晓阳，陈慧泽，韩 榕 *

期 刊 ：西北植物学报 2012年 1期 页码：131~135

关键词：He-Ne激光；拟南芥；种子萌发；幼苗生长；

Keywords:He-Ne laser, Arabidopsis thaliana, seed gernermination, seedling growth,

摘 要 ：以哥伦比亚生态型（Columbia-0）的野生型拟南芥为材料，利用He-Ne激光生物辐照仪(632.8 nm，5 mW/mm ²)处理拟南芥种子,统计其发芽势和发芽率，并测定根长及叶绿素、可溶性糖、可溶性蛋白和丙二醛（MDA）的含量，以探讨He-Ne激光对拟南芥种子萌发和幼苗生长的影响。结果显示，短时间的He-Ne激光辐照可促进拟南芥的生长发育，且最佳辐照时间为4 min，此时的发芽势、发芽率、根长、叶绿素、可溶性糖及可溶性蛋白的含量均达到最大，而丙二醛（MDA）的含量为最低。当辐照时间大于4 min时，促进作用逐渐变小，且随着辐照时间的延长而表现出抑制作用。研究表明，适量的He-Ne激光辐照在一定程度上可以促进拟南芥种子的萌发和幼苗生长以及保护其细胞膜免受伤害。

Abstract:We choose Columbia wild type Arabidopsis thaliana as the experimental material,using He-Ne laser instrument(632.8 nm,5 mW/mm ²) to handle the seeds,then gather statistics about germination potential,germination percentage,root length,the contents of chlorophyll,soluble sugar,soluble protein and MDA.The results showed that,short period of He-Ne laser radiation could promote the growth of A.thaliana.It has an obvious influence when the time reaches 4 minutes,and the germination potential,germination percentage,root length,the contents of chlorophyll,soluble sugar,soluble protein reach the highest.However,the content of MDA reach the lowest.When the time is beyond 4 minutes,the promotion influence become less,and then it inhibited with longer radiation time.All of these show that suitable He-Ne laser dosage radiation could promote the seeds germination and seedling growth of A.thaliana and protect the cell membrane from being injured.

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