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作 者 ：张俊芳1,2，徐 立2，李志英2*

期 刊 ：西北植物学报 2012年 5期 页码：866~870

关键词：蜻蜓凤梨；MADS-box基因；RT-PCR；

Keywords:Aechmea fasciata, MADS-box gene, RT-PCR,

摘 要 ：为探索MADS-box基因在凤梨花发育过程中的调控机制，通过设计简并引物，利用RACE技术，从蜻蜓凤梨花蕾中分离得到2个花发育相关B类MADS-box基因，分别命名为AfAP3和AfPI;AfAP3 cDNA全长957 bp，编码区编码226个氨基酸;AfPI cDNA全长808 bp，编码区编码198个氨基酸，二者均具有典型的植物MADS-box蛋白结构。RT-PCR分析结果表明，AfAP3和AfPI基因主要在花器官中表达，在根系中也有微量表达；乙烯诱导后7 d，AfPI基因在茎尖处开始有表达，表明此时蜻蜓凤梨花芽分化可能已经完成，AfAP3基因表达晚于AfPI。

Abstract:Two flowering related MADS-box genes,named as AfAP3 and AfPI were cloned from flower bud of Aechmea fasciata through designing degenerate primer and RACE technology.The full cDNA fragment of AfAP3 was 957 bp and coded a polypeptide of 226 amino acids.The full cDNA fragment of AfPI was 808 bp and coded a polypeptide of 198 amino acids.Both genes had a typical MADS-box domain.RT-PCR analysis showed that these two genes were main expressed in floral organs;on the other hand,they were also expressed a little in root.After flower induction by exogenous ethylene,AfPI gene began to express in stem tip at 7 d,AfAP3 gene began to express later.

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