---

作 者 ：陈元印1,2，唐运来1,2*，陈 梅1,2

期 刊 ：西北植物学报 2012年 3期 页码：491~497

关键词：小球藻；限氮培养；水分含量；叶绿素荧光参数；

Keywords:Chlorella sorokiniana, nitrogen-limited culture, water content, chlorophyll fluorescence parameters,

摘 要 ：限氮培养是提高小球藻油脂含量的一种方法，本研究探讨了小球藻限氮培养过程中藻细胞的生物量、水分含量和叶绿素荧光参数的变化。结果表明：在限氮培养条件下，小球藻藻细胞的生物量和水分含量分别下降了18%和7%；藻细胞的最大光能转化效率(F_v/F_m)，表观量子效率(α)和最大光合电子传递速率(rETR_max)在整个限氮培养过程中均快速下降，到培养末期下降至接近于0，显著低于对照；半饱和光强(E_k)在对数期迅速下降，到稳定期后显著上升；最小荧光(F₀)在整个限氮培养过程中显著上升。可见，限氮培养显著影响了小球藻细胞光系统Ⅱ的结构和功能。

Abstract:Nitrogen-limited culture is a kind of method improving the oil content of Chlorella sorokiniana.The biomass,water content and fluorescence parameters of chlorella cultivated under nitrogen-limited medium were investigated.The results showed that the biomass and water content of chlorella under nitrogen-limited culture declined 18% and 7%,respectively;the maximal photochemical efficiency of PSⅡ (F_v/F_m),apparent quantum efficiency (α) and maximum photosynthetic electron transport rate (rETR_max) rapidly fell during the course of nitrogen-limited culture,and fell to close zero by the later period of culture;the minimum saturating irradiance (E_k,light-resistant index) firstly rapidly decreased from the early logarithmic period,and then remarkably increased during stable period;the minimum fluorescence(F₀) obviously increased during the nitrogen-limited culture.These results showed that nitrogen-limited culture significantly influenced the structure and function of PSⅡ in chlorella.

---