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作 者 ：段英姿

期 刊 ：西北植物学报 2012年 8期 页码：1534~1538

关键词：菘蓝；二倍体；同源四倍体；遗传差异性；ISSR；

Keywords:Isatis indigotica Fort., diploid, homological tetraploid, genetic variation, ISSR,

摘 要 ：以1个菘蓝二倍体及其10个同源四倍体株系为材料，利用19个随机扩增ISSR引物分析其遗传差异性，为菘蓝多倍体诱变的基因表达调控以及遗传改良提供依据。结果显示:菘蓝二倍体与其同源四倍体及四倍体之间的ISSR多态性有明显差异，除主要遗传位点相同外，有些四倍体株系扩增条带数多于二倍体，有些四倍体株系扩增条带数少于二倍体，四倍体株系间亦有多态位点； 11个株系共扩增111条多态性条带，多态性达55.22%。聚类分析显示，不同四倍体株系与二倍体的遗传差异大小亦不同。研究表明，菘蓝二倍体与其同源四倍体具有中等偏高的遗传差异性。

Abstract:ISSR molecular markers with ninteen primers were used to analyze the genetic variation between 1 diploids and their 10 homological tetraploids of Isatis indigotica Fort..The results showed that genetic variation was distinct difference between diploids and their homological tetraploids of Isatis varied with different genotypes.In addition to the main genetic loci were same,some tetraploids amplified extra bands and some tetraploids with missing bands.There were polymorphic loci between the homological tetraploids.11 strains were detected 111 alleles,its homological tetraploid was 55.22%.The clustering results show that genetic variation is different between diploids and tetraploids.The genetic variation with medium is on the high side between diploids and tetraploids by ISSR.

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