摘 要 ：以模式植物拟南芥为材料,采用RT-PCR分析、基因克隆、转化等方法对CIPK1基因功能进行了研究.结果发现,CIPK1(CBL-interacting protein kinase 1)基因在拟南芥茎和花中表达量最高,在叶中表达量最低;ABA能迅速诱导CIPK1基因的表达,GA则抑制该基因的表达,但2,4-D和6-BA对该基因的表达无明显影响;通过对CIPK1基因转基因突变体进行ABA处理,发现转基因拟南芥种子的萌发率明显高于野生型.以上结果表明CIPK1基因的表达具有组织特异性,并且该基因参与ABA和GA信号传导,尤其在ABA促进种子休眠的信号传导途径中具有非常重要的作用.
Abstract:RT-PCR,gene clone,transformation were used to study the function of CIPK1(CBL-interacting protein kinase 1) gene in Arabidopsis.The result showed that CIPK1 gene expressed at high levels in flowers and stems and at low levels in leaves.Moreover,CIPK1 gene