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作 者 ：康冰 曹建军

期 刊 ：西北植物学报 2002年 6期

关键词：转抗虫基因欧美黑杨；离体快繁技术；新芽增殖；生根培养；

Keywords:Populuas euramericana, insect-resistant transgenic, tissue culture, regeneration, rooting,

摘 要 ：以抗虫欧美黑杨的叶，带腋芽茎段为外植体进行离体快繁技术研究。最佳接种时间为8月份，新芽生长迅速。基本培养基为MS，较适初培养基为MS＋6－BA0.5mg/L（以下单位同）＋NAA0.01mg/，附加30g/L，蔗糖，7g/L琼脂。愈伤组织诱导并同时分化出新芽培养基为MS＋6－BA1.5 NAA0.3，附加40g/L蔗糖，6g/L琼脂。继代增殖培养基为MS 6-BA1.0 NAA0.1 GA2.0，附加30g/L蔗糖，5g/L琼脂。生根培养基为MS＋IBA2．0。

Abstract:Shoots and axillary buds were induced in vitro from apexes,stem segments and leaves of insect-resistant transgenic Populus euramericana .August is the best inoculated time and new buds grew rapidly.Basic medium is MS medium.The proper initial cultu

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