摘 要:以美洲黑杨杂种优良无性系南林 895 杨为转基因受体材料,利用农杆菌介导法转化与逆境胁迫相关的 DREB1C 基因。经潮霉素筛选,共获得 80 株抗性植株,经 PCR 及RT-PCR检测有 30 株抗性植株呈阳性,初步证明 DREB1C 基因已整合到南林 895 杨基因组中。抗性试验结果初步表明,转基因植株的抗旱、耐盐性均有所提高。
Abstract: An elite hybrid clone of Populus×euramericana cv. Nanlin 895 was used as the receptor material for transgenic transformation, and Agrobacteriummediated method was applied to introduce DREB1C gene encoding a transcription factor related with stresses.A total 80 resistant plants were gained via strictly selection by hygromycin. Among them 30 positive transgenic plants were verified by PCR and real-time PCR analysis. It is indicated that DREB1C gene was probably integrated into genome of Nanlin 895. Furthermore, results of resistant experiment showed that resistance of transgenic plants to drought and salt was stronger than non-transgenic plants.
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