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Transformation of Drought and Salt Resistant Gene ( DREB1C ) in Populus×euramericana cv. Nanlin 895

南林 895 杨抗旱耐盐基因 DREB1C 的转化


摘 要:以美洲黑杨杂种优良无性系南林 895 杨为转基因受体材料,利用农杆菌介导法转化与逆境胁迫相关的 DREB1C 基因。经潮霉素筛选,共获得 80 株抗性植株,经 PCR 及RT-PCR检测有 30 株抗性植株呈阳性,初步证明 DREB1C 基因已整合到南林 895 杨基因组中。抗性试验结果初步表明,转基因植株的抗旱、耐盐性均有所提高。

Abstract: An elite hybrid clone of Populus×euramericana cv. Nanlin 895 was used as the receptor material for transgenic transformation, and Agrobacteriummediated method was applied to introduce DREB1C gene encoding a transcription factor related with stresses.A total 80 resistant plants were gained via strictly selection by hygromycin. Among them 30 positive transgenic plants were verified by PCR and real-time PCR analysis. It is indicated that DREB1C gene was probably integrated into genome of Nanlin 895. Furthermore, results of resistant experiment showed that resistance of transgenic plants to drought and salt was stronger than non-transgenic plants.


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