---

作 者 ：戴群 刘秉胜 何放亭 陈子文

期 刊 ：林业科学 1998年 34卷 5期 页码：74-78

关键词：温度；桑树植原体；聚合酶链式反应(PCR)；

Keywords:Mulberry tree phytoplasma, Polymerase chain reaction(PCR), Temperature,

摘 要 ：通过聚合酶链式反应(PCR)检测了植原体在桑树体内的分布及其相对含量的周年变化。结果显示,茎部中植原体的含量在生长季节逐渐升高,7月达到最大,而冬季检测不到,叶部与茎部相似但植原体的含量在8月达到最大;根部全年均含有植原体,含量较低。结果表明,桑树植原体在寄主体内的消长与温度的周年变化有关。

Abstract:By polymerase chain reaction (PCR),the seasonal variation of the phytoplasmal amounts in mulberry tree was determined.It was demonstrated that the phytoplasma was unevenly distributed in the host.In stem,its amount increased during the growing season,which reached its peak in July and was undetected in winter;in leaves,the situation was similar to that in stem but the peak was in August.In roots,its amount was lower and hardly varied in all the year round.It was indicated that the seasonal variation of phytoplasma in mulberry trees was related to that of temperature.

---