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作 者 ：苏平;赵金梅;赵开弘;周明

期 刊 ：武汉植物学研究 2008年 26卷 6期 页码：573-580

关键词：别藻蓝蛋白α亚基；别藻蓝蛋白β亚基；体内重组；聚集态分析；

Keywords:α-APC, β-APC, Reconstitution in vivo, Aggregation,

摘 要 ：为了研究鱼腥藻PCC7120(Anabaena sp.PCC7120)中别藻蓝蛋白(APC)α和β亚基(α-APC和β-APC)中藻蓝胆素(PCB)与脱辅基蛋白的生物合成,并在蓝藻体外对这两种色素蛋白PCB-ApcA和PCB-ApcB合成时聚集过程进行分析,通过多种组合的质粒在大肠杆菌体内共同表达进行重组。色素蛋白的吸收和荧光光谱以及Zn电泳表明,在大肠杆菌体内同时得到色素蛋白PCB-ApcA和PCB-ApcB,并且体内重组色素蛋白的细胞荧光光谱显示,色素蛋白以三聚体的形式存在,而破碎细胞后所得上清液所显示的光谱特征为单聚体的特征。

Abstract:In order to study the biosynthesized α-APC and β-APC,chromoprotein PCB-ApcA and PCB-ApcB was obtained by in vivo reconstitution in Escherichia coli,and was shown through the absorption spectra and the fluorescence spectra.A plasmid containing apcA and apcB was constituted by inserting this two genes in the duet vector with two multiple clone sites separately.Chromoprotein PCB-ApcA and PCB-ApcB could be biosynthesized in vivo simultaneously in E.coli.Through in vivo reconstitution of multiple combinations,(α^APCβ^APC)₃ could be detected in the cell and α^APCβ^APC in the supernatant.

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