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作 者 ：刘凤栾;杨利平;尚爱芹;董胜豪

期 刊 ：武汉植物学研究 2009年 27卷 4期 页码：403-406

关键词：百合；酶活性；膜脂过氧化；电阻率；结实率；远缘杂交；

Keywords:Lilium, Enzyme activity, Membrane lipid peroxidation, Resistivity, Seed setting rate(SSR), Distant cross,

摘 要 ：以百合(Lilium)品种‘Pollyanna’为材料,研究了KCl处理对柱头中SOD、POD及CAT等3种保护酶活性、可溶性蛋白含量以及电阻率的影响。结果表明,处理后3种酶的活性均表现出不同程度的应激性升高,其中SOD活性在花开当天和花开1d显著升高,在蕾期与花开2d极显著升高;花开当天及花开1d的POD活性极显著升高;CAT活性在花开1d升高显著,花开2d及3d时升高极显著。柱头可溶性蛋白含量及电阻率无显著变化,表明KCl处理削弱了细胞膜的膜脂过氧化作用,延缓了柱头衰老,提高了授粉受精作用,且与结实率升高表现出一定相关性。

Abstract:The effects of KCl stress on the activities of protective enzymes(SOD,POD and CAT),the content of soluble protein and resistivity of Lilium‘Pollyanna’stigma were studied.The results showed that the activities of the three enzymes were increased by stress in different degree.Compared to control,the SOD activity increased on bloom day and one day after anthesis and significantly increased on the bud stage and two days after anthesis.The POD activity increased significantly on bloom day and one day after anthesis.The CAT activity increased on one day after anthesis and significantly increased on two and three days after anthesis.But the content of soluble protein and resistivity had no significant difference between the control and the treatments.These indicated that KCl treatments retarded membrane lipid peroxidation,delayed the stigma senescence,improved the pollination and fertilization,which correlated with the seed setting.

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