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作 者 ：陶月红;张昭;张本刚;斯建勇

期 刊 ：武汉植物学研究 2008年 26卷 5期 页码：542-546

关键词：化感物质；化感作用；研究方法；生物检测；定量检测；

Keywords:Allelochemical, Allelopathy, Research-methods, Bioassay, Quantificational-detection,

摘 要 ：在有关化感物质的研究中,由于一些观念和实验方法的限制,对于脂溶性物质在自然生态环境中是否有化感作用存在争议。在化感物质的生物检测中,脂溶性成分的实验方法比较混乱,并存在很多问题,直接影响到实验的准确性和有效性,不能客观科学地进行定性及定量测定。植物的化感作用是在一个复杂的生态体系中发生的,不应主观排除脂溶性物质在自然界中的化感作用,尤其是现在化感作用机理研究越来越成为研究的热点和重点,要阐明化感作用的分子化学、酶调控和基因调节机制,迫切需要建立一个目标植物全成分化感活性筛选的基础平台,并建立定量化感活性生物检测方法。

Abstract:In allelocchemicals researches,the fat-soluble components causing allelopathy in natural ecological environment or not is still controversial as a result of limited concepts and experimental methods.Actually,the current bioassay methods of fat-soluble components have lots of disadvantages,which directly affect the validity and accuracy of allelopathical experiments.Plant allelopathy occurs in the complex ecosystem,we can not ignore the allelopathy of fat-soluble substances subjectively along with allelopathy mechanism is attracting the attention of more and more researchers.Therefore,it is urgent to establish a platform of wide components selection of allelopathy activity to elucidate the allelopathy mechanism of the molecular chemistry,enzyme and gene regulation.

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