Abstract:In this joint paper the authors first report on the results of the G-bands of chromosomes of Lilium davidii, Pinus armardii and . By our observation, G-bands differ from the C-bands observed before. Usually, the chromosomes of these plants mentioned above display 1-4 C-bands, and they are distributed usually in the centromere regions; the G-bands appear as many as tens. All of them are distributed in the entire length of the chromosomes, and are clear enough. G-bands display granular state in the prophase figure, and display the clear bands in the metaphase. These are quite similar to the G-bands in mammalian chromosomes. G-bands number depends on the degree of chromosomal contraction. Band number of prometaphase chromosomes is three times as many as that of metaphase chromosomes, and is near to the human High-Resolution bands level. G-bands were analysed by the automatic spectrum analysis, and the wave crest value conformed with the Gbands. In this paper, the authors introduce the application of trypsin method in G-bands of plant chromosomes. It indicates that this method is not only suitable to animals, but also to plants. However, the key points of G-bands for plant chromosomes seem to be the suitable division phase and the treatment technique of chromosomes rather than the trypsin method itself.