摘 要 ：本文研究了13个栽培大豆（Glycine max L.）品种原生质体培养的再生能力。从大豆幼荚子叶酶解游离原生质体，用Gellan Gum进行株状包埋，悬浮在含2，4-D 0.1-0.2mg/L，BA0.5-1.0mg/L的改良MS液体培养基中，原生质体培养3天后开始第一次分裂，以后持续分裂。供试基因型间的10天植板率差异显著，变幅为33-67%。30天内形成大量的
Abstract:Thirteen soybean (Glycien max L.) genotypes were evaluated for their regenerability from protoplasts. Protoplasts were isolated from immature cotyledons, buried in beads solidified with Gellan Gum, and cultured in modified MS liquid medium containing asparagines, glutamine, allantoin, and allantoic