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作 者 ：杨明挚,陈善娜,郡波,刘继梅,黄兴奇

期 刊 ：云南植物研究 1999年 21卷 02期 页码：1-3

关键词：黑子南瓜；甘油-3-磷酸酞基转移酶；cDNA；

Keywords:Cucurbita ficifolia,

摘 要 ：依据国外报道的南瓜甘油一3一磷酸转酷酶（GPAT）基因的cDNA序列合成相应引物，用 RT－PCR技术，成功地分离了黑子南瓜（Cucurbita ficifolia）GPAT基因的 cDNA片段，并亚克隆到了 pGEM－T载体系统的多克隆位点上，序列分析表明黑子南瓜 GPAT基因的 cDNA序列及递推的氨基酸序列与南瓜（Cucurbita moschata）相比分别具有 98％和 96.5％的同源性。在1188hp中有22个核苷酸发生变化，导致13个氨基酸的改变。

Abstract:According to the cDNA sequence of GPAT from squash ( Cucurbita moschata) reported in 1988, using RT - PCR technical, we isolated the cDNA encoding a glycerol - 3 - phosphate acyltransferase ( GPAT) from figleaf gourd ( Cucurbita ficifolia) cotyledons and subcloned in pGEM - T vector system. The cDNA and the deduced amino acid sequence of figleaf gourd GPAT have 98 and 96.5 homologous with squash respectively. Among the deduced amino acid residues thirteen of them are different from that of squash.

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