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期 刊 ：植物遗传资源学报 2014年 15卷 5期

关键词：关键词异交率；特早熟甘蓝型油菜；综合杂交种；SSR；

Keywords:Key words: outcrossing rate, Brassica napus L., Synthetic hybrids, SSR,

摘 要 ：利用SSR分子标记技术构建3个特早熟甘蓝型油菜恢复系的指纹图谱，筛选出这3个特早熟甘蓝型油菜恢复系的共显性SSR标记，测定了这3个恢复系的异交率。结果表明，恢复系材料4395、3509、4152的异交率分别为46.02%、33.32%、18.12%，在p=0.01时呈极显著差异，说明3个特早熟甘蓝型油菜恢复系的异交差异明显，为确定3个特早熟甘蓝型油菜恢复系在综合杂交种中比例高低提供依据。

Abstract:Abstract:Fingerprinting of three very early Brassica napus restorer lines by using SSR molecular markers . CO dominant SSR markers were found in the 3 early maturing B.napus restorer lines and their F1; Those primers were used to determine the outcrossing rate of these 3 restorer lines in fields. The results showed that the outcrossing rate of these restorer lines (4395, 3509, 4152 ) were respectively in 46.02%, 33.32%, 18.12%, there was a significant difference in p = 0.01, it illustrated that the outcrossing rate of three very early maturing B.napus restorer lines had differences; And It provided a basis that to determine the proportion of three very early Brassica napus restorer lines in Synthetic hybrids.

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