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期 刊 ：植物遗传资源学报 2015年 16卷 5期

关键词：玉米；百粒重；QTL定位；导入系；掖478；

Keywords:maize (Zea mays L.), 100-kernel weight, QTL mapping, introgression line, Ye478,

摘 要 ：玉米百粒重是产量性状的主要组成因子，对其控制位点进行QTL鉴定或基因克隆，将有益于其遗传控制的研究和分子育种的实施。本研究以导入系SL19-41为材料，该导入系是以我国玉米育种中广泛应用的骨干自交系掖478（Ye478）为遗传背景导入QB80染色体片段的纯合系。使用该导入系与Ye478杂交构建分离群体（F2、F2:3家系和BC1F1），通过三个环境下的田间试验，利用Icimapping的逐步回归区间作图法进行百粒重QTL定位，以及进行QTL位点连锁标记的表型效应分析。结果表明：鉴定了2个百粒重QTL位点，其中位于第4染色体bnlg1784～umc1194区间QTL位点qKW4-1在三个环境下均被检测到，可解释的表型变异为6.74%～17.81%，阐明了导入系SL19-41百粒重性状的遗传机制，同时也获得了改良版的Ye478（Ye478QB80），为玉米百粒重的遗传改良提供有益的分子标记，也为克隆百粒重QTL基因提供材料来源。

Abstract:The 100-kernel weight of maize is a main component of yield traits. The research of genetic controlling sites by identifying QTL or cloning genes is helpful for the implementation of molecular breeding. In this study, We used introgressed homozygous line SL19-41, containing two chromosome fragments of QB80 introgressed into the genetic backgrounds of Ye478, because Ye478 is a main maize inbred line with widely used in maize breeding. We constructed the segregated population families by hybridizinng with Ye478 (F2、F2:3 and BC1F1). After the field experiment of three environments, we located the 100-kernel weight QTL and analyzed the phenotypic effects of QTL loci linked markers through the stepwise regression interval mapping by IciMapping software. The results showed that two QTLs of 100-kernel weight were identified, of which qKW4-1 located in bnlg1784-umc1194 interval on the 4 chromosome which are repeatly detected in three environments, interpreting 6.74%-17.81% phenotypic variations and illustrated genetic mechanisms of 100-kernel weight of introgression line SL19-41, At the same time, we obtained the modified version of the Ye478 (Ye478QB80), it provided useful molecular markers for genetic improvement of 100-kernel weight of maize and also provided material resources for cloning genes of 100-kernel weight.

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