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期 刊 ：植物遗传资源学报 2015年 16卷 3期

关键词：水层保湿；普通细菌性疫病；针刺叶片法；资源筛选；

Keywords:storage water, common bacterial blight, needle method, resistant germplasm resources screening,

摘 要 ：采用针刺叶片法接种、温室地面水层保湿方式，对146份普通菜豆种质资源进行抗普通细菌性疫病的鉴定和评价，结果表明，采用的接种方法简便实用，保湿方式效果良好，鉴定结果准确；从146份普通菜豆种质中筛选出抗病种质（R）2份、中抗（MR）种质50份、感病（S）种质81份、高感种质（HS）13份。因此，本文推荐针刺叶片接种、温室地面水层自然蒸发保持湿度可以作为大规模菜豆抗普通细菌性疫病鉴定的适宜的方法。

Abstract:By using ground storage water to moisturize after needle method, 146 accessions were evaluated for resistance to common bacterial blight of common bean in the greenhouse. The results showed that the inoculation was reliable. Combined with moisturizing of ground storage water after inoculation by needle method, we found 2 resistant, 50 moderately resistant, 81 susceptible and 13 high susceptible accessions of germplasm resources. This paper provides a reference method to evaluate resistant resources to common bacterial blight of common bean. The inoculation technique could be used as a basal identification method of seedling stage resistance to common bacterial blight in the greenhouse.

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