---

作 者 ：黄仕周 刘艾琴

期 刊 ：云南植物研究 1986年 8卷 02期 页码：1-3

关键词：快速微型繁殖；实生成年苹果树；

Keywords:Rapid micro-propagation,

摘 要 ：试验结果表明：以实生成苹果树上的芽作为外植体，培养在添加1-2mg/1 6-BA的SH或MS培养基上，在继代培养时芽的萌发率达92%以上，50天内芽数增殖5-6.8倍；同期内，液体旋转培养比琼脂静置培养芽条长度要增加倍左右；芽条在0.2-0.4mg/1IBA中生根率80%以上；试管苗的移栽成活率85%以上。

Abstract:The experimental reslllt showed: In the mcdium of Murashigr and Shoog (MS) or Schenk and Hidebrandt (SH) added with 6-benzylamino-purine (6 -BA) at 1- 2 mg/l, the sprouting rate of buds in subculture of the buds from the adult seedling app1e trees were over 92%. Buds multiplied 5-6.8 time in fifty days. The length of shoots in a rotating liquid culture was about three times more than that in the culture on agar-medium. In the medium added with indole- 3 -butyric acid (IBA) at 0.2-0.4 mg/l, the ro-oting rate of shoots were over 80%. The suryiyal rate of transplanting tuble- plantlets were more than 85%.

---