---

期 刊 ：植物遗传资源学报 2015年 16卷 2期

关键词：野油胞质；雄性不育系 1193A；恢复基因；SSR标记；

Keywords:Sinapis arvensis L cytoplasm, Male sterile line 1993A, restore gene, SSR markers,

摘 要 ：以野油胞质雄性不育系 1193A 和恢复系 1193R2 为亲本构建 F2 分离群体，并运用 BSA 法构建了可育和不育基因池。利用 1521 对SSR 引物进行了多态性分析，结果表明有 36 对引物在亲本和基因池间都表现多态性，用 F2 单株验证表明有 11 对引物与恢复基因连锁，离恢复基因较近的两个标记 CB10316 和 BnGMS171，分布在恢复基因 Rf 的两侧，遗传距离分别为 3.9cM 和 5.7cM，可作为恢复系标记辅助育种的候选标记。

Abstract:In order to study restoring gene, F2 population was builded by male sterile line 1193A with Sinapis arvensis L cytoplasm as female parent and restorer line 1193R2 as male parent. Using SSR marker and bulked segregant analysis (BSA) , polyacrylamide gel electrophoresis were performed to identify genetic linkage markers for 1193A CMS restorer gene and to map the markers. 1521 SSR markers were analyzed and the result showed 36 markers were polymorphic between parents and gene bulks. F2 population detection indicated 8 markers linked to the restore gene, two of which located on both sides of the restore gene and closely linked to the restore gene with a genetic distance of 3.9 cM for CB10316 and 5.7 cM for BnGMS171, respectively, which could be used as candidate markers for marker-assisted breeding of restorer line.

---