作 者 ：江伟,胡永敏

期 刊 ：中国中药杂志 2011年 36卷 7期 页码：926.

关键词：健脾益气清幽汤；慢性浅表性胃炎；淋巴细胞亚群；IL-2 mRNA；

Keywords:Jianpi Yiqi Qingyou decoction, chronic superficial gastritis, lymphocyte subsets, IL-2 mRNA,

摘 要 ：目的: 探讨健脾益气清幽汤对慢性浅表性胃炎大鼠胃组织淋巴细胞亚群及IL-2 mRNA的影响。 方法 : 将66只Wistar大鼠随机分为空白对照组、模型组、健脾益气清幽汤低、中、高剂量组及兰索拉唑组, 用0.02%氨水给大鼠自由饮用,连续90 d,制备大鼠试验性浅表性胃炎动物模型。用HE染色检测动物造模成功,免疫组化检测胃组织CD3⁺,CD4⁺,CD8⁺T细胞。RT-PCR检测胃组织IL-2 mRNA表达。 结果 : 健脾益气清幽汤高剂量组大鼠胃组织CD3⁺,CD4⁺T细胞数量明显升高,CD8⁺T细胞数量明显下降,与模型组比较有显著性差异(P<0.01)。高、中剂量组大鼠胃组织IL-2 mRNA表达明显增多,与模型组比较有显著性差异(P<0.01)。 结论 :健脾益气清幽汤能明显提高胃组织淋巴细胞CD3⁺,CD4⁺T细胞数量及IL-2 mRNA表达,降低CD8⁺T细胞数量,提高大鼠免疫功能,为慢性浅表性胃炎的致病机制及治疗提供了依据。

Abstract:Objective : To investigate the effect of Jianpi Yiqi Qingyou decoction on lymphocyte subsets and IL-2 mRNA in gastric tissue in rats with chronic superficial gastritis. Method : Wistar rats were randomly divided into 6 groups (11 for each): a blank control groups, the model of the control groups, the treatment groups (low-dose groups of traditional Chinese medicine, moderate-dose groups of traditional Chinese medicine, high-dose groups of traditional Chinese medicine) and lansoprazole groups. The models were made with the method in reference except a blank control groups. These rats are drinking freely with 0.02% ammonia, continuous 90 days, and made preparations for experimental animal model of superficial gastritis. Making the model were detected by HE dying. The count of CD3⁺, CD4⁺ and CD8⁺ T cells were detected by immunohistochemistry.Using reverse transcriptase polymerase chain reaction (RT-PCR), the expression levels of IL-2 mRNA in gastric tissue were quantified. Result : Compared with that in model groups, the content of CD3⁺ T cells and CD4⁺T cells in gastric tissue obviously increased in high dose of traditional Chinese medicine groups , the content of CD8⁺ T cells in gastric tissue obviously decreased in high dose of traditional Chinese medicine groups and the difference was significant(P<0.01) .The expression levels of IL-2 mRNA in gastric tissue obviously increased in moderate and high doses of traditional Chinese medicine groups, and the difference was significant compared with that in model group(P<0.01) . Conclusion : Jianpi Yiqi Qingyou decoction can obviously improve the content of CD3⁺ T cells and CD4⁺ T cells and the expression levels of IL-2 mRNA, decrease the content of CD8⁺ T cells in gastric tissue, improve immunity of rats. So the research results can provide some evidences for the treatment for chronic superficial gastritis.