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作 者 ：范秀华,卢雯平,魏晗,李向英,蔡建辉

期 刊 ：中国中药杂志 2009年 34卷 23期 页码：3119.

关键词：疏肝益肾方剂；Ishikawa细胞；雌激素受体α；雌激素受体β；顺铂；

Keywords:traditional Chinese drugs shuganyishen, Ishikawa cells, estrogen receptor α, estrogen receptor β, cisplatin,

摘 要 ：目的：研究疏肝益肾方剂对体外Ishikawa细胞的作用及意义。方法：采用中药血清药理学方法制备大鼠含疏肝益肾方剂血清（含药血清组），并体外作用Ishikawa细胞，同时设正常细胞组（阴性对照组）、大鼠阴性血清对照组（阴性血清组）、顺铂组（阳性对照组）和疏肝益肾方剂联合顺铂组（联合用药组），MTT检测各组对Ishikawa细胞株的抑制率、RT-PCR检测各组雌激素受体α（estrogen receptor α，ERα）、雌激素受体β（estrogen receptor β，ERβ）的转录水平。结果：MTT结果显示含药血清组、联合用药组与阴性对照相比均对Ishikawa细胞株有抑制作用（P＜0.05），而与阳性对照组相比无统计学差异。RT-PCR结果显示含药血清组与阴性对照相比可轻微升高Ishikawa细胞株中ERβ的转录水平，对ERα的转录水平无明显影响；联合用药组则降低ERα的转录水平（P＜0.05）、降低ERβ作用不明显；而阳性对照组降低ERα，ERβ作用均显著（P＜0.05）。结论：中药疏肝益肾方剂对体外Ishikawa细胞株有抑制作用，并轻微提升ERβ的表达，但几乎不影响ERα的表达。

Abstract:Objective： To study the effects of the traditional Chinese drugs，Shuganyishen，on Ishikawa cell line. Method： Rat blood serum was prepared by using Chinese drugs serum pharmacology method，in which，Shuganyishen was contained or not contained. Ishikawa cells were subjected into five groups： ① normal cells group (NCG)，②rat blood serum without Shuganyishen group (NSGG) as negative control，③Shuganyishen group(SGG)，④Cisplatin group (DDPG) as positive control，and ⑤Shuganyishen with Cisplatin group (S&CG). MTT assay was used to evaluate the inhibition effect on cell growth in each group. The mRNA expression of estrogen receptor α and β (ERα and ERβ) in each group was detected by RT-PCR. Result： Effective inhibition of cell growth was found in the groups of SGG and S&CG，respectively (P＜0.05)，which had no significant difference from positive control by MTT. The mRNA expression of ERβ was slightly going up，while the expression of ERα was hardly effected in SGG by RT-PCR. Interestingly in S&CG，the mRNA expression of ERα was significantly down-regulated (P＜0.05)，but the mRNA expression of ERβ remained no change. As the positive control，the mRNA expression of ERα and ERβ was significantly down-regulated in DDPG (P＜0.05). Conclusion： Traditional Chinese drugs，Shuganyishen，may inhibit Ishikawa cell growth and had no effects on the expression of ERα，furthermore，can reverse the inhibition of ERβ expression by Cisplatin.

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