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作 者 ：王群,刘继虹,王国宏,吴波,胡兰萍,李洁,孙玲君,王坚

期 刊 ：中国中药杂志 2010年 35卷 12期 页码：1612.

关键词：模拟航渡；大强度运动；三七总皂苷；神经-内分泌-免疫网络；

Keywords:experimental navigation, intensive exercise, Panax notoginseng saponins (PNS), neuroendocrine-immune network,

摘 要 ：目的: 观察模拟航渡及大强度运动对雄性大鼠神经-内分泌-免疫网络的影响及三七总皂苷的调控作用。 方法 : 将6周龄的SD雄性大鼠随机分为静息对照组(QC组)、训练对照组(TC组)和三七总皂苷组(PNS组),每组10只。QC组不接受任何刺激,在生理盐水灌胃1周后即进行取样。TC组和PNS组大鼠经过10 d递增负荷跑台训练,分别生理盐水和PNS灌胃1周后,进行模拟航渡及高强度运动,结束即刻取血,使用放免法测定大鼠血清皮质酮(Cort)、促肾上腺皮质激素(ACTH)、β-内啡肽(β-EP)、神经肽Y(NPY)、白介素1β(IL-1β)和白介素6(IL-6);采用免疫组织化学方法检测各组大鼠垂体ACTH, NPY的表达水平,并进行相关分析;电镜下观察垂体、肾上腺超微结构。 结果 :训练对照组大鼠与静息对照组相比,血清Cort, β-EP水平显著升高,同时,垂体ACTH, NPY表达水平显著降低。灌服PNS后与TC组相比,血清Cort, ACTH及NPY水平下降;同时,免疫组化结果表明垂体ACTH, NPY表达水平也明显增高。在此基础上观察到应激后大鼠腺垂体及肾上腺皮质的超微结构改变,功能受损,灌服PNS后对细胞具有保护作用。 结论 : 负性心理应激及大强度的运动训练使大鼠神经-内分泌-免疫网络功能紊乱,PNS对神经-内分泌-免疫网络具有调控作用。

Abstract:Objective : The present study aims to study the influences experimental navigation and intensive exercise on immune-neuroendocrine network of the male rats and the effects of PNS to this influence. Method : Thirty 6-week Sprague-Dawley male rats (SD rats) were randomly llocated into three groups: Quiescent control (QC) group, training control (TC) group and Panax notoginseng saponins (PNS) group. Rats from QC group were not given any stimuli, and samples were taken after 7-day intragastric administration of saline. Rats from TC group underwent 10-day run training of increasing load on treadmill and received 7-day intragastric administration of saline. PNS group were subjected to the same procedure of run training as group TC, and received intragastric administration of PNS at the dose of 0.2 g·kg^-1. Blood samples were immediately obtained at the end of the tests to determine the serum levels of corticosterone (Cort), adrenocorticotropic hormone (ACTH), beta-endorphin (β-EP), Neuropeptide Y (NPY) and interleukin-1β (IL-1β), interleukin-6(IL-6) by RIA. Expressions of Pituitary ACTH, NPY were observed use the immunohistochemistry method and correlation analyses conducted. And the ultrastructural changes of the pituitaries and the adrenal cortex cells were examined by electron microscope meanwhile. Result : The serum β-EP and Cort levels in TC group were significantly increased compared to the QC group; whereas Expression levels of pituitary ACTH, NPY were markedly higher after experimental navigation and intensive exercise. In Group PNS, the plasma Cort, ACTH and NPY levels decreased significantly compared to Group TC. Meanwhile we found expression levels of pituitary ACTH, NPY also lower than group TC. There was no significant difference about the ultrastructure of anterior pituitary and adrenal cortex of QC and PNS group. However, a obvious change of ultrastructure occurred to TC group rats. Conclusion : These results suggest that immune-neuroendocrine network function of rats were confused by negative psychological stresses and intensive exercise. PNS therapy may exert regulation effects to the network.

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