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期 刊 ：中国中药杂志 2007年 32卷 11期 页码：1060.

关键词：天麻糖蛋白PGE2-1；抗血凝；抗血栓；

Keywords:PGE2-1, anticoagulation, antithrombosis,

摘 要 ：目的：观察天麻糖蛋白(PGE2-1)的抗血凝与抗血栓作用。方法：玻片法、减尾法检测小鼠凝血时间(CT)和出血时间(BT)；减尾测5 min后的出血量吸光度(A₅₄₀)；小鼠体内测定血浆复钙时间(RT)；ADP诱导血小板聚集(PAG)；试剂盒测定血浆凝血酶原时间(PT)、凝血酶时间(TT)、部分凝血活酶时间(APTT)；体外血栓仪测定大鼠体外形成血栓的长度、湿重、干重；实验性动静脉旁路血栓模型测定血栓的湿重及PGE2-1对其的抑制率。结果：60,120 mg·kg^-1的PGE2-1能显著延长小鼠的凝血时间、出血时间,增大出血量(A540)(P<0.05或P<0.01)。30,60,120 mg·kg^-1的PGE2-1能延长小鼠血浆复钙时间,降低血小板聚集率(P<0.05或P<0.01)。10,20,40 mg·mL^-1的PGE2-1能使TT和APTT显著延长(P<0.05或P<0.01),但对PT的影响无统计学意义。30,60,120 mg·kg^-1的PGE2-1能使小鼠消除血栓症状、恢复自主活动时间明显缩短(P<0.05或P<0.01)；使大鼠体外形成的血栓长度显著减短、湿重和干中显著减小(P<0.05或P<0.01)；极显著减小大鼠实验性动脉血栓的湿重(P<0.01),各药物组抑制率分别为32.5%,49.0%和61.5%。结论：PGE2-1具有显著的抗凝、抗栓作用,可能为天麻提取物在抗栓方面的主要成分。

Abstract:Objective: To investigate the effects of polysaccharide 2-1 from Gastrodia elata (PGE2-1) on blood coagulation and thrombosis. Method: Clotting time (CT) and bleeding time (BT) of mice were measured by glass method and tail-cutting method.Bleeding capacity (A₅₄₀) was measured by cutting tail in 5 min.Plama recalcificatic time (RT) were measured in mice.Platelet aggregation was caused by adenosine diphosphate (ADP).Activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) were measured by reagent boxes.During thrombosis in vitro, their lengths, wet and dry weights were measured by instrument; wet weights of arteriovenous experimental thrombosis were measured and the impressive rates were analyzed. Result: CT and BT of groups PGE2-1 (60, 120 mg·kg^-1) were remarkably prolonged, and bleeding capacity (A₅₄₀) were significantly increased (P<0.05 or P<0.01). RT of groups PGE2-1 (30, 60, 120 mg·kg^-1) were remarkably prolonged, and platelet aggregation (PAG) were inhibited (P<0.05 or P< 0.01). Human serous TT and APTT of groups PGE2-1 (10, 20, 40 mg·mL^-1) were remarkably prolonged (P<0.05 or P<0.01), but the difference of effect on PT had no statistic significance. PGE2-1 (30, 60, 120 mg·kg^-1) could make the mice obviously eliminate thrombus symptom and reduce the time of restoring independent activity (P<0.05 or P<0.01); thrombosis in vitro: Lengths, wet and dry weights of groups PGE2-1 (30, 60, 120 mg·kg^-1) were significantly decreased (P<0.05 or P<0.01); wet weights of arteriovenous experimental thrombosis were dramatically decreased (P<0.01), and impressive rates were respectively 32.5%, 49.0% and 61.5%. Conclusion: PGE2-1 has remarkable effects of anticoagulation and antithrombosis, so it may be the main component of the isolation from G. elata in the field of antithrombosis.

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