Abstract:Objective: To establish a system of cell suspension culture for Saussurea involucrate. Method: The effects of different factors on cell growth and flavonoids production of S. involucrate were systematically studied including the media, initial pH values of the medium, carbon sources, inoculum quantity, and plant growth substance. Result: The optimum medium was N6, initial pH values of the medium was5. 8, sucrose concentration was 50g·L-1, inoculum quantity was 60-80 g·L-1 FW. Medium supplemented with BA (0.5mg·L-1) and NAA(3 mg·L-1) was suitable for cell growth, but medium containing BA (0. 2 mg·L-1) and NAA(2 mg·L-1) was suitable for flavonoids production. Conclusion: Cell growth and flavonoids production in the suspention of S. involucrate culture cell should be optimized.