Abstract:Objective: To investigate the chemical constituents in Flos Sophorae Carbonisatus. Method: SiEca gel column chromatog-raphy was used to separate and purify the chemical constituents. The structures were elucidated by spectral analysis. Result: Six compounds were isolated from Flos Sophorae Carbonisatus, and their structures were elucidated as maltol (1) , 3-hydroxypyridine (2) , malto-3-O-[6′-O-(4″-hydroxy-trans-cinnamoyll-β-D-glucopyranoside (3), 3-O-[β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranosyl] sophoradiol ethyl ester (4), 3-O-[β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranosyl] sophoradiol methyl ester (5), rutin (6). Conclusion: 4 is a new compound, and 1,2,3,5 were first reported from Flos Sophorae Carbonisatus.