Abstract:Objective: To investigate the chemical constituents of Tetrastigma hemsleyanum. Method: Compounds were separated by rechromatography on silica gel from the chloroform solubles of its ethanol extracts, and the structures were determined by spectral analysis and chemical evidence. Result: Three compounds were isolated and elucidated as 6′-O-benzoyldaucosterol, daucosterol and β-sitosterol.Conclusion: All the three compounds were isolated from this plant for the first time, of which 6′-O-benzoyldaucosterol is a new natural product.