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期 刊 ：中国中药杂志 1998年 23卷 12期 页码：726.

关键词：魔鬼爪浸膏；帕歌斯风湿乐片；哈帕甙；薄层鉴别；薄层扫描；

Keywords:Devil Claw Extract, Pagosidel Devil Claw Extract tablets, harpagoside, TLC, TLC scanning,

摘 要 ：目的:对魔鬼爪浸膏(Devil’sClawExtract)及制剂帕歌斯风湿乐片(PagosidDevil’sClawEx-tractTablets)中的环烯醚萜甙类成分进行定性鉴别,并定量测定其主要活性成分哈帕甙(harpagoside)。方法:硅胶预制薄层板,氯仿-乙酸乙酯-甲醇-水为展开剂,5%香草醛 硫酸溶液或10%硫酸-乙醇溶液为显色剂做为定性鉴别;并采用薄层扫描法测定哈帕甙的含量,以氢氧化钠碱性板展开后,于283nm紫外吸收扫描。结果:线性范围16.8ng～151.2ng,平均回收率为101.2%。结论:本方法经线性关系、灵敏度、专属性、稳定性及重现性方法考察证明符合定量要求。

Abstract:Objective:To set up a method for TLC identification of the Devil Claw Extract and its tablets,and make a quantitative analysis of harpagoside the main active constituent in the Extract.Method:A characteristic TLC chromatogram was obtained by using pre coated silica gel 60F₂₅₄plate,chloroform ethyl acetate methanol water as the solvent system and 5% vanillin sulfuric acid solution or 10% sulfuric acid ethanol solution as the spray reagent;and quantitative analysis was carried out using silica gel plate modified by 1% sodium hydroxide,and UV absorptive scannig at 283nm.Result:The regression equation is Y=20.33X+92.72,r=0.9993,linear range between 16.8～151.2ng,and average recovery 101.2%.Conclusion:This method has been validated by linearity,stability,sensitivity and reproducibility tests,and proved to be accordant with the requirement for quantitative analysis of the herbal medicine in question.

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