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期 刊 ：中国中药杂志 1996年 21卷 05期 页码：299.

关键词：人参总甙；绞股蓝总甙；钠 .钾腺苷三磷酸酶；微粒体；

Keywords:ginsenoside, gypenoside, sodium, potasium adenosine triphosphatase, microsomes,

摘 要 ：对照人参总甙（Ｇｉｎ）观察绞股蓝总甙（Ｇｙｐ）对大鼠心、脑微粒体ＡＴＰ酶活性的影响。结果表明Ｇｙｐ体外显著抑制心、脑Ｎａ^＋，Ｋ^＋－ＡＴＰ酶，并具浓度依赖性。其ＩＣ_５０分别为５８．７９±８．０５，５２．０７±６．２５ｍｇ／Ｌ。提示Ｇｙｐ的强心、中枢抑制作用可能与对Ｎａ^＋，Ｋ^＋－ＡＴＰ酶的抑制有关。

Abstract:The effects of gypenoside(Gyp) on the microsomal Na⁺,K⁺-ATPase from rat hearts and brains were investigated in comparison with ginsenoside(Gin).The results showed that Gyp could inhibit the enzyme ac-tivity rapidly and reversibly in vitro.The inhibition was in a concentration-depend manner.The IC₅₀of Gyp for the heart and brain was 58.79±8.05mg/Land52.07±6.25mg/L respectively.Kinetic analysis revealed that Gyp was an incompetitive inhibitor of ATP.These results suggest that the positive inotropcefect and the inhibition of CNS by Gypare related to the in hibitory action of Gyp on the actiyily of Na⁺,K⁺-ATPase from hearts or brains.

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