Abstract:From the roots of Bupleurum longicaule var. franchetii. B. chaishout and The official species B. chinense, ten saikosaponins have been isolated and identified as saikosaponin a, saikosaponin d, saikosaponin c, saikosaponin e. saikosaponin b2, 2"-O-acetylsaikosaponin a, 3"-O-acetylsaikosaponin a. 3"-O-acetylsaikosaponin d, 6"-O-acetylsaikosaponin a and 6"-O-acetyltsaikosaponin d respectively. It has been shown that the chemical constituents of these three Bupleurum plants are very similar, therefore, the medicinal value of B. longicaule var.franchetii and B.chaishoui is further verified.