Abstract:In this paper the relationship of Tibetan Huanglian and Yunnan Huanglian with Coptis from has been studied. The distribution and taxonomic features of Tibetan Huanglian are just the same as Coptis from, but quite different from Yunnan Huanglian. The results reveal that Tibetan Huanglian and Yunnan Huanglian are different in botanical origin.