全 文 ：广 西 植 物 Guihaia Jul． 2013，33(4) :523－527 http:/ / journal． gxzw． gxib． cn
DOI:10． 3969 / j． issn． 1000-3142． 2013． 04． 017
何双辉，李海蛟． 中国锈革菌属研究． 5． 采自云南的两个新记录种［J］． 广西植物，2013，33(4) :523－527
He SH，Li HJ． Hymenochaete in China． 5． Two new records from Yunnan Province［J］． Guihaia，2013，33(4) :523－527
Hymenochaete in China． 5． Two new
records from Yunnan Province
HE Shuang-Hui*，LI Hai-Jiao
(Institute of Microbiology，Beijing Forestry University，Beijing 100083，China )
Abstract:Hymenochaete contiformis and H． rufomarginata，collected from Yunnan Province，are newly recorded in Chi-
na． While Hymenochaete contiformis is characterized by its anatomical structure (without a cortex and hyphal layer) ，
long setae and large basidiospores;H． rufomarginata is distinguished by its presence of numerous hyphidia and both
small setae and basidiospores． Illustrated descriptions of these two species based on the Chinese materials and an identi-
fication key to all the 30 Hymenochaete species in Yunnan Province are provided．
Key words:Hymenochaete contiformis;H． rufomarginata;Hymenochaetaceae;wood-inhabiting fungi;taxonomy
中图分类号:Q949 文献标识码:A 文章编号:1000-3142(2013)04-0523-05
中国锈革菌属研究． 5． 采自云南的两个新记录种
何双辉* ，李海蛟
(北京林业大学 微生物研究所，北京 100083 )
摘 要:报道了采自云南省的锈革菌属(Hymenochaete)2个中国新记录种，即长矛锈革菌(H． contiformis)和红边
锈革菌(H． rufomarginata)。前者的主要特点是解剖结构中无皮层和菌丝层，刚毛长且孢子大;后者的主要特点
是子实层具大量的侧丝，刚毛和孢子较小。提供了这两个种的野外标本照片、显微结构绘图以及详细的形态学
描述，并给出了云南省锈革菌属 30个种的检索表。
关键词:长矛锈革菌;红边锈革菌;锈革孔菌科;木生真菌;分类
Wood-inhabiting fungi are very important in the
forest ecosystem because of their ecological functions
and economic values (Wei ＆ Dai，2004;Dai et al．，
2007;Dai ＆ Yang，2008)． The diversity of wood-inhabi-
ting fungi in Yunnan Province is extremely high，and
many new species and Chinese new records of polypores
have been found in this area (Cui et al．，2011)． The
corticioid genus Hymenochaete (Basidiomycota，Hym-
enochaetales)in the province has also been studied by
several authors (Xu et al．，2003;Zhang ＆ Dai，2005) ，
however，its diversity in this area is not well known． In
2011，two intensive surveys of Hymenochaete in Yunnan
Province were carried out，and more than 200 specimens
were collected． Two new species and three Chinese new
records have been found and published from these spec-
imens in previous studies (He ＆ Li，2011，He ＆ Dai，
2012)． Ｒecently，two additional species，Hymenochaete
contiformis and H． rufomarginata were identified as new
to Chinese fungal flora，and till now，30 species of Hym-
enochaete have been found in Yunnan Province． Illus-
收稿日期:2012-11-22 修回日期:2013-03-29
基金项目:国家自然科学基金 (31000006)
作者简介:何双辉，博士，副教授，主要从事木生真菌分类与分子系统学研究，(E-mail)shuanghuihe@ yahoo． cn。
* 通讯作者 (Author for correspondence)
trated descriptions of the two species based on Chinese
materials and an identification key to all the 30 Hym-
enochaete species in Yunnan Province are provided in the
present paper．
1 Materials and Methods
Voucher specimens are deposited in the herbarium
of Beijing Forestry University (BJFC) ，and the micro-
scopic procedure follows Zhao ＆ Cui (2011)． In pres-
enting the size range of spores and setae，5% of the
measurements were excluded from each end of the
range，and the measurements were given in parentheses．
In the text the following abbreviations were used:L =
mean spore length，W=mean spore width，Q = variation
in the L /W ratios between the specimens studied，n=the
number of spores measured from given number of speci-
mens． IKI stands for Melzer’s reagent，KOH for 5% po-
tassium hydroxide，and CB is the abbreviation of Cotton
Blue in lactic acid． IKI-= inamyloid and indextrinoid，
CB-=acyanophilous．
2 Ｒesults and Analysis
Hymenochaete contiformis G． Cunn．，Trans． Ｒoy．
Soc． N． Z． 85:41，1957．(Fig． 1-2)
Fruitbody．—Basidiocarps annual，effused，closely
adnate，coriaceous，first as small colonies，later confluent
up to 8 cm or more in longest dimension，80-200 μm
thick in section． Hymenophore smooth，grayish brown to
clay-buff，usually not cracked;margin thinning out，dis-
tinct， fimbriate，paler than hymenophore surface，
cinnamon to yellowish brown when juvenile，becoming
indistinct，concolorous with age．
Hyphal structure．—Hyphal system monomitic;gen-
erative hyphae without clamp connections;tissue darke-
ning but otherwise unchanged in KOH．
Subiculum．—Tomentum，cortex and hyphal layer
absent．
Stratified hymenium．—Generative hyphae hyaline
to yellowish brown，thick-walled，agglutinated，2-3 μm in
diam． Setal layer composed of 1-3 rows of overlapping
setae． Agglomerates of crystals frequently present in the
setal layer and subhymenium，up to 40 μm in diam． Se-
tae numerous，subulate，reddish brown，with acute tips，
Fig． 1 A basidiocarp of Hymenochaete
contiformis (He 1166)
Fig． 2 Microscopic structures of Hymenochaete
contiformis (drawn from He 1166)
a． Basidiospores;b． Basidia and basidioles;c． Setae．
usually enmeshed with a thin hyphal sheath，projecting
up to 120 μm above the hymenium，(70-)90-160 μm×
(7-)9-14(-15)μm． Cystidia absent，simple hyphidia
present，not numerous． Basidia clavate，with four sterig-
425 广 西 植 物 33卷
mata and a simple septum at base，18-22 μm×4-6 μm;
basidioles in shape similar to basidia，but smaller．
Spores．—Basidiospores ellipsoid to cylindrical，hy-
aline，thin-walled，smooth，IKI-，CB-，(7． 6-)7． 8-9． 5
(-10)μm×(3． 2-)3． 5-4． 2 μm，L = 8． 68 μm，W=3． 83
μm，Q=2． 18-2． 36 (n=60 /2)．
Specimens examined．—China． Yunnan Prov．，
Chuxiong，Zixishan Forest Park，on fallen angiosperm
twig，16． IX． 2011 He 1083;Weixi County，Pantiange，
on fallen angiosperm trunk，22． IX． 2011 He 1166．
Ｒemarks．—Previously，Hymenochaete contiformis
was reported in Brazil，Costa Ｒica，Jamaica，New
Zealand，Ｒéunion and Venezuela (Léger，1998)． It is
characterized by its anatomical structure (without a
cortex and hyphal layer) ，long setae and large basidio-
spores (Cunningham，1957;Léger，1998)． H． contiformis
is very similar to H． longispora Parmasto，but the latter
has paler basidiocarps and lacks agglomerates of crystals
in the setal layer and subhymenium (Léger，1998;He ＆
Li，2012)．
Hymenochaete rufomarginata Imazeki，Bull． Tokyo
Sci． Mus． 2:9，1940．(Fig． 3-4)
Fig． 3 Basidiocarps of Hymenochaete
rufomarginata (He 619)
Fruitbody．—Basidiocarps annual，effused or
effused-reflexed with slightly elevated margins，loosely
adnate，easily detachable from the substrates，coriaceous
to woody hard，brittle，reflexed part projecting up to 0． 2
cm，resupinate part up to 10 cm or more in longest di-
mension，up to more than 1 mm thick in section． Hym-
enophore smooth，fawn to cinnamon，not cracked;margin
thinning out，paler than hymenophore surface，cinnamon
Fig． 4 Microscopic structures of Hymenochaete
rufomarginata (drawn from He 619)
a． Basidiospores;b． Basidia and basidioles;c． Setae;
d． Hyphidia;e． Hyphae from subiculum．
to cinnamon-buff，distinct，up to 1． 5 mm wide，becoming
concolorous，indistinct with age．
Hyphal structure．—Hyphal system subdimitic;gen-
erative hyphae without clamp connections;tissue darke-
ning but otherwise unchanged in KOH．
Subiculum．—Tomentum，cortex and hyphal layer
present． Cortex composed of strongly agglutinated
hyphae，50-80 μm thick． Generative hyphae hyaline to
yellowish brown， thin-to thick-walled， septate，
moderately branched，2-3 μm in diam． Skeletal hyphae
distinctly thick-walled with a narrow lumen，not
branched，2-2． 8 μm in diam．
Stratified hymenium．—Hyphae in this layer similar
to those in subiculum，yellowish brown，thick-walled，ag-
glutinated，2-2． 5 μm in diam． Setal layer thickening，
composed of several rows of overlapping setae． Setae
numerous，subulate or fusoid，reddish brown，with acute
tips，usually enmeshed with a thin hyphal sheath，pro-
jecting up to 25 μm above the hymenium，(25-50)μm×
5254期 何双辉等:中国锈革菌属研究． 5． 采自云南的两个新记录种
(5-8)μm． Cystidia absent，hyphidia present，numerous，
yellowish brown，with thickened walls，simple or
branched，sinuous． Basidia clavate，with four sterigmata
and a simple septum at base，(12-18)μm×(3-3． 8)μm;
basidioles in shape similar to basidia，but smaller．
Spores．—Basidiospores ellipsoid to ovoid，hyaline，
thin-walled，smooth，IKI-，CB-，(2． 5-)2． 8-3． 5(-3． 8)
μm×2-2． 5(-2． 8)μm，L=3． 06 μm，W=2． 22 μm，Q=
1． 38 (n=30 /1)．
Specimens examined．—China． Yunnan Prov．，Pu’
er County，Caiyanghe Nat． Ｒes．，on fallen angiosperm
trunk，6． VI． 2011 He 619;Yingjiang County，Tongbiguan
Nat． Ｒes．，on fallen angiosperm trunk，29． X． 2012 He
1471，30． X． 2012 He 1478 ＆ He 1480;Longchuan
County，Tongbiguan Nat． Ｒes．，on fallen angiosperm
trunk，31． X． 2012 He 1489 ＆ He 1492． Japan，Mont
Takatori-Yama，Naka-Gun，Kanagawa Pref．，13． XI． 1932
TNS-F 203362 (holotype)．
Ｒemarks．—Previously，Hymenochaete rufomarginata
was only reported in Japan，and no basidiospore was ob-
served from the types． The main characters distinguishing
H． rufomarginata from other species of the genus are its
anatomical structure (with a cortex and hyphal layer)，
small setae and presence of numerous hyphidia (Imazeki，
1940;Léger，1998)． The Chinese specimen has a few ba-
sidiospores and fits well the description of Imazeki
(1940)and Léger (1998)except for the thicker basidio-
carps． H． rufomarginata shares similar anatomical struc-
tures，small setae and simple or branched hyphidia with
H． dura Berk． ＆ M． A． Curtis，but the latter has slightly
longer basidiospores (4-5 μm，Léger，1998)．
30 key species of Hymenochaete so far found
in Yunnan Province
1． Hymenophore lamellate or poroid 2……………………………
1． Hymenophore smooth 3…………………………………………
2． Hymenophore lamellate H． cyclolamellata……………………
2． Hymenophore poroid H． xerantica……………………………
3． Hyphidia numerous 4…………………………………………
3． Hyphidia absent or rare 10…………………………………
4． Hyphidia acanthoid H． murina……………………………
4． Hyphidia not acanthoid 5…………………………………
5． Hyphidia dendroid 6………………………………………
5． Hyphidia not dendroid 7…………………………………
6． Dendrohyphidia arbusculoid;tropical species H． floridea………
6． Dendrohyphidia not arbusculoid;temperate species
H． sphaericola
……………
…………………………………………………
7． Hyphidia simple，never branched 8……………………………
7． Hyphidia usually branched 9…………………………………
8． Basidiocarps resupinate;hyphidia smooth H． innexa…………
8． Basidiocarps effused-reflexed or pileate;hyphidia encrusted
H． luteobadia
…
…………………………………………………
9． Cortex present，setae (25-50)μm×(5-8)μm;basidiospores
(2． 8-3． 5)μm×(2-2． 5)μm H． rufomarginata……………
9． Cortex absent，setae (50-90)μm×(7-11)μm，basidiospores
(3． 2-5)μm×(1． 8-2． 8)μm H． tropica……………………
10． Basidiocarps effused-reflexed or pileate 11………………
10． Basidiocarps strictly resupinate 19………………………
11． Cortex absent 12………………………………………
11． Cortex present 15………………………………………
12． Basidiospores ＜ 3． 5 μm long 13………………………
12． Basidiospores ＞ 3． 5 μm long 14………………………
13． Setal layer with horizontal agglutinated hyphae
H． subferruginea……………………………………
13． Setal layer without horizontal agglutinated hyphae
H． adusta……………………………………………
14． Basidiocarps effused-reflexed，woody hard;setal layer
thickening H． unicolor………………………………
14． Basidiocarps pileate，coriaceous;setal layer not
thickening H． rheicolor……………………………
15． Setae encrusted with crystals;basidiospores
＞ 7 μm long H． megaspora………………………
15． Setae smooth，basidiospores ＜ 7 μm long 16………
16． Setal hyphae present;hyphae and hymenial cells
encrusted H． yunnanensis………………………
16． Setal hyphae absent;hyphae and hymenial cells
smooth 17…………………………………………
17． Basidiospores ＞ 4 μm long H． rubiginosa………
17． Basidiospores ＜ 4 μm long 18…………………
18． Submonilioid hyphal ends present in hymenium
H． villosa………………………………………
18． Submonilioid hyphal ends absent in hymenium
H． ochromarginata……………………………
19． Setae with 1-3 teeth H． separabilis…………
19． Setae without teeth 20………………………
20． Setae blunt，spathulate H． spathulata………
20． Setae acute，not spathulate 21………………
21． Setae with encrustation 22…………………
21． Setae smooth 26……………………………
22． Setae encrusted with crystals 23…………
22． Setae encrusted with yellowish brown
resinous granules 24…………………
625 广 西 植 物 33卷
23． Basidiospores subglobose H． sphaerospora……………………
23． Basidiospores cylindrical H． legeri……………………………
24． Hyphal layer absent;setae ＜ 50 μm long;basidiospores
＜ 4． 5 μm long H． minor……………………………………
24． Hyphal layer present;setae ＞ 50 μm long;basidiospores
＞ 4． 5 μm long 25…………………………………………
25． Basidiospores (4． 8-6)μm×(2． 3-3． 3)μm
H． rhabarbarina
…………………
………………………………………………
25． Basidiospores (6-7)μm×(4-5)μm H． rhododendricola……
26． Cortex present H． fulva……………………………………
26． Cortex absent 27…………………………………………
27． Basidiospores ＞ 7 μm long 28……………………………
27． Basidiospores ＜ 7 μm long 29……………………………
28． Crystals present in setal layer and subhymenium
H． contiformis…………………………………………
28． Crystals absent H． longispora…………………………
29． Setae (70-120)μm×(5-9)μm;basidiospores (4． 5-6． 5)
μm×(1． 8-2． 8)μm H． cinnamomea…………………
29． Setae (30-45)μm×(5-7)μm;basidiospores (3． 2-4)μm×
(1． 8-2． 3)μm H． anomala……………………………
Ｒeferences:
张小青，戴玉成． 2005． 中国真菌志第 29卷—锈革孔菌科［M］．
北京:科学出版社:1－205
Cui BK，Du P，Dai YC． 2011． Three new species of Inonotus (Ba-
sidiomycota，Hymenochaetaceae)from China［J］． Mycol Prog，
10:107－114
Cunningham GH． 1957． Thelephoraceae of New Zealand XIV-The
genus Hymenochaete［J］． Trans Ｒoy Soc New Zeal，85:1－51
Dai YC． 2010． Hymenochaetaceae (Basidiomycota)in China［J］．
Fung Div，45:131－343
Dai YC(戴玉成) ，Yang ZL(杨祝良)． 2008． A revised checklist
of medicinal fungi in China(中国药用真菌名录) ［J］． Mycosys-
tema(菌物学报) ，27:801－824
Dai YC，Cui BK，Yuan HS，Li BD． 2007． Pathogenic wood-
decaying fungi in China［J］． For Path，37:105－120
He SH，Dai YC． 2012． Taxonomy and phylogeny of Hymenochaete
and allied genera of Hymenochaetaceae (Basidiomycota) in
China［J］． Fung Div，56:77－93
He SH，Li HJ． 2011． Hymenochaete in China． 2． a new species
and three new records from Yunnan Province［J］． Mycotaxon，
118:411－422
He SH(何双辉) ，Li HJ(李海蛟)． 2012． Two species of Hym-
enochaete new to China(锈革菌属两个中国新记录种) ［J］．
Guihaia(广西植物) ，32:19－22
Imazeki Ｒ． 1940． Studies in the genus Hymenochaete of Japan［J］．
Bull Tokyo Sci Mus，2:1－22
Léger JC． 1998． Le genre Hymenochaete Léveillé［J］． Biblioth My-
col，171:1－319
Wei YL(魏玉莲) ，Dai YC(戴玉成)． 2004． The ecological
function of wood-inhabiting fungi in forest ecosystem(木材腐朽
菌在森林生态系统中的功能) ［J］． Chin J Appl Ecol(应用生
态学报) ，15:1 935－1 938
Xu SZ(徐士忠) ，Zhou TX(周彤燊) ，Wang L(王琳) ，et al．
2003． A note on the species and new records of Hymenochaete
Lév in Yunnan(云南锈革菌属真菌及新记录种) ［J］． J South-
west For Coll(西南林学院学报) ，23:53－58
Yuan HS，Dai YC． 2008． Polypores from northern and central Yun-
nan Province，southwestern China［J］． Sydowia，60:147－159
Zhao CL(赵长林) ，Cui BK(崔宝凯)． 2011． Two species of ligni-
colous fungi new to China (中国木生真菌两新记录种) ［J］．
Guihaia(广西植物) ，31:721－724
櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔
(上接第 578页 Continue from page 578 )
chrysum arenarium［J］． Chin Pharm J，43(1) :11－13
Markham KＲ，Ternai B． 1976． Flavonoids other then flavone and
flavonol aglycones［J］． Tetrahedron，32(21) :2 607－2 612
Wang KJ(王开金) ，Chen LZ(陈烈中) ，Li N(李宁) ，et al． 2006．
Antioxidant and radical-scavenging activity of flavonoids from Sol-
idago canadensis(加拿大一只黄花黄酮成分及抗氧化与自由基
消除活性研究) ［J］． Chin Pharm J(中国药学杂志) ，41(7) :
493－496
Wen DX(文东旭) ，Zheng XZ(郑学忠) ，Shi JX(史剑侠) ，et al．
1999． Studies on the chemical constituents of triquetrous tadehagi
(Tadehagi triquetrum) (Ⅰ) (葫芦茶化学成分的研究Ⅰ) ［J］．
Chin Trad Herb Drugs(中草药) ，30(4) :252
Wen DX(文东旭) ，Lu MY(陆敏仪) ，Tang ＲJ(唐人九) ，et al．
2000． Studies on the chemical constituents of Triquetrous tadehagi
(Tadehagi triquetrum) (Ⅱ) (葫芦茶化学成分的研究Ⅱ) ［J］．
Chin Trad Herb Drugs(中草药) ，31(1) :3
Wei X，Ｒong TL，Yun LM，et al． 2005． Four new prenylated isofla-
vonoids in Tadehagi triquetrum［J］． J Agric Food Chem，53(2):267
Yves C，Gilles C，Joseph V，et al． 1999． Norterpenoid and sesquiter-
penoid glucosides from Juniperus phoenicea and Galega officinalis
［J］． Phytochemistry． 50:1 219－1 223
Zhang LH(张雷红) ，Zhang XT(张现涛) ，Ye WC(叶文才) ，et al．
2006． Chemical constituents from Euphorbia latifolia(宽叶大戟
化学成分的研究) ［J］． Nat Prod Ｒes Develop(天然产物研究与
开发) ，18:58－60
Zhou XD(周旭东) ，Shi LY(史丽颖) ，Yu DY(于大永) ，et al．
2010． Effect of active part extracted from Tadehagi trquetrum
(Linn．)Ohashi on Type I allergy induced by IgE(葫芦茶抗 IgE
介导 I型抗过敏有效部位的研究) ［J］． Central South Pharm
(中南药学) ，9(1) :35－38
7254期 何双辉等:中国锈革菌属研究． 5． 采自云南的两个新记录种