摘 要 :以巨峰、玫瑰香、龙眼和泽香4个葡萄品种一年生扦插苗为试材,研究了氯化镉(CdCl2)处理后葡萄根系线粒体过氧化氢(H2O2)、膜通透性转换孔(MPTP)、膜电位(Δψ)、细胞色素C(Cyt c)及根系活力的变化.结果表明:4个葡萄品种经0.5 mmol·L-1的CdCl2处理后,根系线粒体H2O2含量上升,MPTP开放程度增大,Δψ降低,Cyt c含量下降,根系活力明显降低;其中,H2O2含量和Cyt c 含量由高到低的顺序为:巨峰>泽香>玫瑰香>龙眼,而根系活力、MPTP和Δψ由高到低的顺序为:龙眼>玫瑰香>泽香>巨峰.表明巨峰葡萄根系活力比其他品种更易受到CdCl2的影响,而龙眼葡萄根系活力在4个品种中受CdCl2影响最小.
Abstract:Taking the one-year cutting-seedlings of four grape cultivars (Kyoho, Muscat Hamburg, Long Yan, and Ze Xiang) as test materials, their root mitochondr ial hydrogen peroxide (H2O2) content, membrane permeability transition pore (MPTP), membrane potential (Δψ), cytochrome C (Cyt c) content, and root activity were measured under effects of CdCl2. For all test grape cultivars, the root mitochondrial H2O2 content and MPTP increased, but the Δψ, Cyt c content, and root activity decreased after treated with 05 mmol CdCl2·L-1. The m itochondrial H2O2 content and Cyt c content were in the sequence of Kyoho>Ze Xiang>Muscat Hamburg>Long Yan, while the root activity, MPTP, and Δψ were in ad verse, i.e., Long Yan>Muscat Hamburg>Ze Xiang>Kyoho. Among the test grape cultiv ars, the root activity of Kyoho was easier to be inhibited under Cd stress, while t hat of Long Yan was least affected by CdCl2.