Abstract:SSR markers were used to study the genetic variation of 16 varieties of sweet corn. First of all, 10 primers were screened with stable polymorphism from the 200 pairs of SSR primers；By the 10 primers, 254 allelic variations were detected in the material, each primer pair with 5 to 38 allele average. The average information quantity of polymorphism was 0.920 8. Accordingly, 16 varieties were divided into three broad categories. The study showed that SSR markers can be used to genetic analysis and heterosis breeding.