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作 者 ：李亚兰, 张全伟, 王雪莹, 马友记, 张勇, 赵兴绪

期 刊 ：生物技术通报 2014年 0卷 5期 页码：102-106

关键词：天祝白牦牛；胰岛素生长因子2；cDNA末端快速扩增；基因克隆；RT-PCR；

Keywords:Tianzhu white yak, IGF-2 Gene, Rapid amplification of CDNA ends(RACE), Gene Clone, RT-PCR,

摘 要 ：旨在克隆天祝白牦牛胰岛素样生长因子2(IGF-2)基因编码区全长cDNA序列，为研究该基因的生理功能奠定基础。运用cDNA末端快速扩增(RACE)技术获得天祝白牦牛IGF-2基因全长cDNA序列。扩增获得天祝白牦牛IGF-2基因全长cDNA序列为1 060 bp(GenBank登录号：KF682139)，ORF长540 bp，编码179个氨基酸。其编码的氨基酸与已报道哺乳动物IGF-2氨基酸序列同源性在80%-92%之间。 天祝白牦牛IGF-2基因的成功克隆为进一步研究该基因的功能奠定了基础。

Abstract:It was to clone insulin-like growth factor2(IGF-2)full length cDNA in Tianzhu white Yak, thus provide a theoretical basis tostudyits mechanisms. IGF-2 gene full length cDNA were cloned by rapid amplification of cDNA ends(RACE). The result showed that the full length of IGF-2 cDNA was 1 060 bp(Gene Bank accession No：KF682139), with an ORF 540 bp in length, which encoded 179 amino acids. The amino sequence analysis indicated that IGF-2 gene in Tianzhou white yak is highly homologous in most mammals. The full length cDNA of 1 060 bp IGF-2 gene was successfully cloned, which laid a basis for further studying of its roles in whit yak.

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