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作 者 ：Lenka Franková and Stephen C. Fry^*

Keywords:Glass-fiber blotting, plant cell wall, polysaccharides (donor), radiolabeled oligosaccharides (acceptor), transglycanase,

Abstract:

Transglycanases (endotransglycosylases) cleave a polysaccharide (donor-substrate) in mid-chain, and then transfer a portion onto another poly- or oligosaccharide (acceptor-substrate). Such enzymes contribute to plant cell-wall assembly and/or re-structuring. We sought a general method for revealing novel homo- and hetero-transglycanases, applicable to diverse polysaccharides and oligosaccharides, separating transglycanase-generated ³H-polysaccharides from unreacted ³H-oligosaccharides—the former immobilized (on filter-paper, silica-gel or glass-fiber), the latter eluted. On filter-paper, certain polysaccharides [e.g. (13, 14)-β-d

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