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作 者 ：戴若兰 张玮 邢更生 李杉 王亚馥

期 刊 ：植物学通报 2000年 17卷 01期 页码：80-83

关键词：枸杞；体细胞胚；DNA合成；立体计量；

摘 要 ：本文以宁夏枸杞无菌苗叶片为材料，离体培养，并诱导体细胞胚胎发生。根据细胞形态计量学原理，应用数字图像处理软件计量由光学底片经A/D转换成的数字图像中的DNA大分子，对枸杞体细胞胚发生过程中DNA分子的代谢动态进行量化分析。结果表明：在整个体细胞胚发生过程中DNA代谢呈现动态变化。非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%；在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长，随着胚性愈伤组织的发育，DNA的含量在梨形胚时期达到高峰；成熟胚的DNA含量虽有所下降，但仍维持较高水平。因此DNA的合成动态变化与体胚生长发育和细胞增殖密切相关。

Abstract:Using leaves of Lycium barbarum L. as explant to induce somatic embr yogenesis. The DNA in somatic embryos of Lycium barbarum L. were ana lysed using quantitative stereological procedure formed from cell morphometry. The results indicated that the quantity of DNA changes during somatic embryogenesis. The quantitive value of DNA was 1.82% and 1.92% respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shap ed embryonic stage keep high level at mature embryonic stage. We concluded that dynamic changes of DNA has close relationship with embryogenesis and cell reproduction.

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