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作 者 ：曹有龙 许兴 宋玉霞 曲玲 罗青

期 刊 ：植物学通报 2001年 18卷 05期 页码：605-614

关键词：枸杞；胚性细胞悬浮系；原生质体；植株再生；

摘 要 ：由枸杞髓部组织诱导出胚性愈伤组织，并由此愈伤组织建立起稳定的细胞悬浮系。从悬浮细胞游离的原生质体在改良KM培养基(1.5 mg/L 6_BA，0.5 mg/L NAA和0.5 mg/L 2,4_D)中进行液体浅层培养，3～4 d后出现第一次分裂，第7 d统计分裂频率为50.3%，15 d左右可形成细胞团，3～4周后形成肉眼可见的愈伤组织，愈伤组织植板率为1.25%。将细胞团转移到液体分化培养基(MS+6_BA 1.5 mg/L+2,4_D 0.2 mg/L) 8～10 d可形成大量胚状体，及时将胚性愈伤组织块转移到固体分化培养基上(MS+6_BA 0.2 mg/L)，可形成大量绿芽，分化率54.17%。绿芽在生根培养基（MS+NAA 0.2 mg/L）可形成完整植株，移栽后成活良好。

Abstract:A stable cell suspension culture was established with callus that was induced from the ripe pith of Lycium barbarum L. The protoplasts were cultured in KM liquid medium supplemented with 1.5 mg/L 6_BA, 0.5 mg/L NAA and 0.5 mg/L 2,4_D. The protoplasts started to divide after 3～4　days. The frequency determined at 7 day s was 50.3%, and cell colonies were formed after about 15 days. Microcalli could be observed in 3～4 weeks. The plating efficiency was 1.25%. Eight to seven days after the microcalli were transferred into the liquid proliferation medium (MS+1.5 mg/L 6_B+0.2 mg/L 2,4_D), a large amount of embryoids appeared on the calli. The embryonic calli were then moved on to a solid medium (MS+ 6-BA 0.2 mg/L) and green buds appeared. The frequency of shoot formation was 54.17%. The shoot rooted in the rooting medium (MS+NAA 0.2 mg/L) and the whole plants were transplanted into pots and grew well.

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